Hi everyone, 

In this video I’ll be talking about the Horvath epigenetic clock, and what may be a possible way of resetting it. First the usual reminder:


This article is intended solely for informational purposes. It is not meant to be taken as, and should not be construed, as medical advice. Any changes to your lifestyle or diet should be done in consultation with your doctor or health care professional.

A recent study suggests that taking five compounds together can turn back Horvath’s epigenetic clock. (In this video I’m calling the compounds “the Horvath cocktail.”) The ingredients are:

  1. human growth hormone (HGH)
  2. metformin
  3. DHEA
  4. vitamin D3
  5. zinc

In theory, resetting the clock could add healthy years to a person’s life. If you turn the clock back two years, according to Dr. David Sinclair, you might (possibly) (theoretically) live two years longer as a result.

Some researchers, such as Peter Attia, are dubious. Attia points out that the study had only nine participants and had poor controls.

And at least one of the components they were taking, synthetic hGH, can be dangerous, according to some studies.

Nonetheless, some folks in the anti-aging community have been looking into whether it might be possible to emulate the study on their own.  

One way of doing so would be to get prescriptions for hGH and metformin from your doctor –– if he or she is willing to prescribe them –– and take the other compounds as supplements. 

Or you could try substituting berberine for metformin (they lower blood glucose in similar ways); taking the zinc, vitamin D, and DHEA as supplements; and focus on raising your hGH by non-pharmaceutical means, which do not appear to have the same negative effects.


One method of raising human growth hormone is fasting. Even skipping dinner (not eating before bed) regularly will raise your HGH levels somewhat.

As a bonus, doing so also raises NAD – nicotinamide adenine dinucleotide – another compound that appears to have anti-aging benefits.

Another method is supplementation, not with hGH, but with amino acids which stimulate the body’s production of hGH. One study found that you can raise your levels significantly by taking these two supplements together:

  • 1500 mg lysine
  • 1500 mg arginine

I found this to be a fascinating study. Subjects were given lysine and arginine in the amounts shown above, with and without exercising. Their blood levels of hGH were tested 30, 60 and 90 minutes later. 

  • At 60 minutes, their hGH levels were “significantly elevated” –– but only in the group that had not exercised. Exercise actually negated the benefits of supplementation, in this study.
  • I tried taking lysine and arginine together but found it hard to fit them into my eating and fasting schedules, which are designed to increase AMPK most days and activate mTOR only in a very limited window.
  • Instead, I’m now doing ALTERNATE DAY FASTING. I fast all day on Monday, Wednesday and Friday, and eat meals on Tuesday, Thursday and Saturday. (Sunday’s a wild card, I can either fast or eat.) 
  • (If you want to try something similar, but aren’t into all-day fasting,  you could try doing modified ADF instead, which allows people to eat one 500 or 600 calorie meal on your “fasting” days.)
  • When you fast, your HGH levels skyrocket. By 36 hours (the start of a feasting day), your HGH levels should be up around 200-300 percent. So it’s an ideal time to add in the other components.
  • On my FASTING days, I take berberine, vitamin D, zinc and DHEA on my FASTING days.
  • On my FEASTING days, lately, I’ve been taking chromium picolinate instead of berberine, because chromium appears to support muscle growth and may also raise testosterone levels. Then I work out a couple of hours later.


  • I’ve been doing the above for a few weeks. I’ve recently added in one more anti-aging component. On feasting days, I also take AAKG (arginine AKG).
  • AKG is also associated with both anti-frailty and anti-aging effects. It raises mTOR, which I’m more interested in doing when I’m feasting than when I’m fasting.


  1. True alternate day fasting (as contrasted to modified ADF). I don’t eat at all on Mondays, Wednesdays or Fridays. The result should be higher hGH.
  2. I take my supplements on fasting days with a small amount of celery juice. It has almost zero carbs, no fat and no protein so I’m not worried about it breaking my fast.
  3. I’ve tried DHEA cream and DHEA capsules. Both work well for me. I get a little jolt of energy when taking or applying DHEA to my skin.
  4. On feasting days (Tuesday, Thursday and Saturday) I add AAKG to the mix, then I wait a little while, have a high-protein meal.

I was already taking many of these supplements for other reasons –– i.e., taking berberine to control blood glucose –– so for me this was more a matter of changing the timing of the supplements than taking something new.

So far I notice a boost in my energy, both physical and mental, particularly on the days when I add in the AAKG.

I should note that these are not the only anti-aging supplements I’m taking; I also take stacks of NAD boosters and testosterone boosters most days. 

I take the NAD boosters in the morning; take the Horvath cocktail an hour or two later; and take T boosters an hour or two later.

Please note that this is not a controlled experiment, just an N=1 exploration to see what happens.  I have no way of knowing for sure whether any effects I end up experiencing are due to this protocol. 

Your thoughts are welcome

I’d be curious what you think about the information in this video. If you feel like it, I’d invite you to type in your comments below.

If you found it interesting, and would like to support my doing future videos, one way to do so is to hit the like button below. This lets Youtube know you liked it, which helps others find the videos. To be notified of future videos, hit subscribe.

I should mention that this channel is currently sponsored by a company called Donotage. They’re a good source for berberine, one of the supplements I take on my fasting days. I’m also using their NMN and resveratrol as NAD boosters. I’ll do a separate NMN and resveratrol soon.

If you decide to buy from Donotage, feel free to use the links below this video. On their checkout page there’s a place to enter a discount code.  If you type in the word PATHWAYS, it’ll will save you 10 percent, a pretty significant savings.


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